35 Evaluation of chromatin and DNA integrity of ejaculated spermatozoa in couples with unexplained recurrent spontaneous abortion

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P-74: Cytochemical Evaluation of Sperm Chromatin and DNA Integrity in Couples with 3 Unexplained Recurrent Spontaneous Abortion

Background: The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. Materials and Methods: In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and cont...

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Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions.

The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and control groups respectively. The semen ...

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Analysis of chromatin integrity and DNA damage of buffalo spermatozoa

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

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P-157: TLR5 Gene Expression in Endometrium of Women with Unexplained Recurrent Spontaneous Abortion

Background: Recurrent spontaneous abortion (RSA) is usually defined as three or more consecutive pregnancy losses before 20th week of gestation. Although different factors are considered as etiology of RSA but in some cases, despite of extensive work up, the cause of RSA remains unknown which called unexplained RSA. Immunological factors are suggested as etiological factors of unexplained RSA. ...

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Comparing Serum Levels of Th17 and Treg Cytokines in Women with Unexplained Recurrent Spontaneous Abortion and Fertile Women

Background: Increased evidences have shown that unexplained recurrent spontaneous abortion (URSA) is associated with inflammatory responses and breakage of immunological autotolerance. Therefore, the balance between Th17 and Treg cells may elucidate the pathophysiology of URSA. Objective: To investigate the serum concentration of regulatory and inflammatory cytokines associated with Treg and Th...

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ژورنال

عنوان ژورنال: Reproductive BioMedicine Online

سال: 2011

ISSN: 1472-6483

DOI: 10.1016/s1472-6483(11)60052-7